The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast obtained from a 60-year-old woman; cells were reportedly tumorigenic in athymic mice and were found to be susceptible to mouse mammary tumor virus.
Tissue of Origin
Homo sapiens (human)
STR DNA Profile
Penta E: 5
Penta D: 9,14
mode = 55; range = 50 to 112; bimodal shift 58 - 59 and 100 in later passages with 3 marker chromosomes
Plasmotest: negative; Mycoplasma specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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