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BHK-21 [C-13];BHK-21

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Cat.No.
CSC-C2006
Description
BHK-21 is a subclone (clone 13) of the parental line established from the kidneys of five unsexed, one-day-old hamsters in 1961; cells can be used for virus replication studies. The cells are positive for porcine circovirus (PCV) antigens. The cells are positive for keratin by immunoperoxidase staining.
Organism
Mesocricetus auratus ,hamster, Syrian golden
Karyotype
Near-diploid golden (Syrian) hamster karyotype with 21% tetraploidy - 40-45, XY - hyperdiploid sideline present at 19%-resembles published description
Source
Kidney, normal
Culture Properties
monolayer
Morphology
fibroblastoid
Recommended Medium
EMEM and 10% h.i. FBS
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6247W
CSC-6248W
CSC-C1081
CSC-C1087
CSC-C1088
CSC-C1105
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