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ActoFactor™ Recombinant Rat Pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
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Cat.No.
CSC-CTK0839
Description
NAP-2 (CXCL7) is a CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is produced in leukocytes by enzymatic processing of a precursor called platelet basic protein (PBP). NAP-2 chemoattracts and activates neutrophils. It is a protein that is released in large amounts from platelets following their activation. It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. Recombinant rat NAP-2 produced in E. coli is a single, non-glycosylated polypeptide chain of 62 amino acids and a molecular mass of 6.8 kDa. It has been purified by proprietary chromatographic techniques.
Species
Rat
Product Overview
Rat Ppbp expressed in E.coli
Molecular Mass
76.8 kDa
Size
CAT# CSC-CTK0839-10 (10 μg); CAT# CSC-CTK0839-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
NAP-2 is fully biologically active when compared to a standard. Biological activity was determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CXCR2. The ED50 for this effect is less than 10 ng/ml, corresponding to a specific activity of Greater than1 x 10^5 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
White (freeze-dried) powder. Lyophilized from a sterile-filtered (0.2 μm) concentrated protein solution in 1×PBS (pH 7.4).
Reconstitution
Please centrifuge product before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa NAP-2 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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