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ActoFactor™ Recombinant Rat Leptin

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Cat.No.
CSC-CTK0874
Description
Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiologic regulation of body weight, leptin has a variety of other physiological and pathological functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant Rat Leptin produced in E. coli is a single, non-glycosylated, polypeptide chain of 147 amino acids and a molecular mass of 16,240 Dalton.
Species
Rat
Product Overview
Rat Lep expressed in E.coli
Molecular Mass
16.24 kDa
Size
CAT# CSC-CTK0874-10 (10 μg); CAT# CSC-CTK0874-50 (50 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Activity
Recombinant rat Leptin is fully biologically active when compared to standards. The ED50, calculated by the leptin-dependant stimulation of Human OB-R transfected murine BaF3 indicator cells, is 0.35-0.06 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered white, lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO3.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa Leptin should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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