Interleukin-5 (IL-5), also known as eosinophil differentiation factor (EDF), is a lineage-specific cytokine for eosinophilpoiesis. It regulates eosinophil growth and activation, and thus plays an important role in diseases associated with increased levels of eosinophils, including asthma. IL-5 has a similar overall fold to other cytokines (e.g., IL2, IL4 and GCSF), but while these exist as monomeric structures, IL-5 is a homodimer. The fold contains an anti-parallel 4-alpha-helix bundle with a left handed twist, connected by a 2-stranded anti-parallel beta-sheet . The monomers are held together by 2 interchain disulphide bonds. Recombinant rat IL-5 produced in E. coli is a dimeric, non-glycosylated polypeptide chain containing 113 amino acids and having a molecular mass of 13,074 Dalton.
Rat Il5 expressed in E.coli
CAT# CSC-CTK0819-10 (10 μg); CAT# CSC-CTK0819-50 (50 μg)
Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Recombinant rat IL-5 is fully biologically active when compared to a standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of BCL-1 cells was found to be < 0.15 ng/ml.
Less than 1 EU/μg.
Sterile-filtered white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) solution in water with no additives.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa IL-5 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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