FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant rat FGF-acidic is a 15.9 kDa protein consisting of 141 amino acid residues.
Rat Fgf1 expressed in E.coli
CAT# CSC-CTK0805-50 (50 μg); CAT# CSC-CTK0805-250 (250 μg)
Greater than 95% as determined by SDS-PAGE and HPLC analysis.
Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is ≤0.1 ng/ml, in the presence of 10 μg/ml heparin, corresponding to a specific activity of Greater than 1 x 10^7 units/mg.
Less than 1 EU/μg.
Sterile-filtered through a 0.2 micron filter and ly ophilized from 10 mM sodium phosphate (pH 7.2), 150 mM NaCl, 0.2% CHAPS.
Please centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term, storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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