ActoFactor™ Recombinant Mouse Vascular endothelial growth factor A (Insect)

Specification
Recommended Products

Cat.No.

CSC-CTK0721

Description

VEGF-164 is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF-164 have been identified, VEGFR-1 (flt-1), and VEGFR-2 (Flk-1). Consistent with the endothelial cell-specific action of VEGF-164, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extravillous trophoblasts. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo and is also a chemoattractant for monocytes and endothelial cells. At least three different proteins are generated by differential splicing of the mouse VEGF gene: VEGF-120, VEGF-164 and VEGF-188. The most abundant form is VEGF-164. Whereas VEGF-120 and VEGF164 are secreted proteins, VEGF188 is strongly cell-associated. In additionn, the isoforms VEGF-164 and VEGF-188 bind to heparin with high affinity. VEGF is apparently a homodimer, but preparations of VEGF show some heterogenity on SDS gels depending of the secretion of different forms and the varying degrees of glycosylation. All dimeric forms possess similar biological activities. There is evidence that heterodimeric molecules between the different isoforms exists and that different cells and tissues express different VEGF isoforms. A related protein of VEGF is placenta growth factor (PlGF) with about 53% homology and VEGF-B with similar biological activities. Mouse Vascular Endothelial Growth Factor 164 (VEGF-164), a 24 kDa protein consisting of 164 amino acid residues, is produced as a homodimer.

Species

Mouse

Product Overview

Mouse Vegfa expressed in Insect cells

Molecular Mass

48 kDa

Size

CAT# CSC-CTK0721-20 (20 μg); CAT# CSC-CTK0721-100 (100 μg)

Expression System

Insect cells

Purity

Greater than 95% as determined by SDS-PAGE analysis.

Activity

The ED50 for stimulation of 3H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF-164 has been determined to be in the range of 1-2 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a protein solution in 50 mM acetic acid with no additives.

Reconstitution

Please centrifuge product briefly before opening the vial. The lyophilized mouse VEGF should be reconstituted in sterile PBS or medium to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu VEGF should be stored long-term in undiluted working aliquots at –20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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