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ActoFactor™ Recombinant Mouse Tumor necrosis factor

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Cat.No.
CSC-CTK0052
Description
Tumor Necrosis Factor - alpha (TNF-a) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes, endothelial cells, smooth muscle cells. TNF-a occurs as a secreted, soluble form and as a membrane anchored form, both of which are biologically active. TNF-a is a pleiotropic factor activating multiple signal transduction pathways and inducing or suppressing the expression of a wide variety of genes. TNF-a plays a critical role in mediation of the inflammatory response and in influencing resistance to infections and tumor growth. It is a trimeric protein that binds to two types of receptor TNF-R1 (p55) and TNF-R2 (p75). Recombinant mouse TNF-a is a 17 kDa protein containing 157 amino acid residues.
Species
Mouse
Product Overview
Mouse Tnf expressed in E.coli
Sequence
A DNA sequence encoding mature soluble mouse TNF-a was expressed in E. coli.
Size
CAT# CSC-CTK0052-10 (10 μg); CAT# CSC-CTK0052-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The biological activity of recombinant mouse TNF-a was measured in a cytotoxicity assay using the TNF-sensitive L-929 cell line in the presence of the metabolic inhibitor, actinomycin D. The ED50 for this effect is typically 0.02-0.05 ng/mL.
Endotoxin Level
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/ml of the cytokine.
Storage & Stability
The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted mouse TNF-a can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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