Thrombopoietin (Tpo) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. Thrombopoietin has been shown to stimulate the maturation, as well as the proliferation, of megakaryocytes. Mouse Tpo is a 35 kDa protein containing 335 amino acid residues. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of approximately 75 kDa in SDS-PAGE.
Mouse Thpo expressed in NSO
A DNA sequence encoding the mouse Tpo was expressed in the mouse myeloma cell line, NSO.
CAT# CSC-CTK0065-5 (5 μg); CAT# CSC-CTK0065-25 (25 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
The ED50 as measured in a cell proliferation assay using MO7e cells is typically 1-3 ng/ml.
Endotoxin level is less than 0.1 ng per mg of mouse Tpo as determined by the LAL method.
Lyophilized from a sterile-filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 5 mg/ml of the cytokine.
Storage & Stability
The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted mouse Tpo can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
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