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ActoFactor™ Recombinant Mouse Thrombopoietin

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Cat.No.
CSC-CTK0065
Description
Thrombopoietin (Tpo) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. Thrombopoietin has been shown to stimulate the maturation, as well as the proliferation, of megakaryocytes. Mouse Tpo is a 35 kDa protein containing 335 amino acid residues. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of approximately 75 kDa in SDS-PAGE.
Species
Mouse
Product Overview
Mouse Thpo expressed in NSO
Sequence
A DNA sequence encoding the mouse Tpo was expressed in the mouse myeloma cell line, NSO.
Size
CAT# CSC-CTK0065-5 (5 μg); CAT# CSC-CTK0065-25 (25 μg)
Expression System
NSO
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The ED50 as measured in a cell proliferation assay using MO7e cells is typically 1-3 ng/ml.
Endotoxin Level
Endotoxin level is less than 0.1 ng per mg of mouse Tpo as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 5 mg/ml of the cytokine.
Storage & Stability
The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted mouse Tpo can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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