Mouse Sonic Hedgehog (Shh-N) is an important signaling molecule expressed during embryonicdevelopment. It is involved in regulating the patterning of the developing central nervous system, somite and limb. mShh may also play a role in the development of whisker, hair, foregut, tooth and bone in the mouse embryo.
Mouse Shh-N expressed in E.coli
A DNA sequence encoding amino acid residues Cys 25-Gly 198 of mouse Shh fused to a 6X histidine tag at the carboxy-terminus was expressed in E. coli.
CAT# CSC-CTK0074-25 (25 μg); CAT# CSC-CTK0074-125 (125 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was measured by its ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts. The ED50 for this effect is 0.6 – 3 mg/mL.
Endotoxin level is less than 0.1 ng per μg of the cytokine as determined by the LAL method.
Lyophilized from a 0.2 μm filtered solution in PBS containing 5% Trehalose and 50mg of bovine serum albumin per 1mg of cytokine.
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 50 μg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Upon reconstitution, mouse Shh-N can be stored under sterile conditions at 2-4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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