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ActoFactor™ Recombinant Mouse Platelet factor 4

  • Specification
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Cat.No.
CSC-CTK0754
Description
Platelet Factor-4 is a 70 amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin A is a member of the CXC chemocinfamily. Human PF-4 is used for the proof of heparin-induced thrombocytopenia. Furthermore, it is used as an inhibitor of angiogenesis during tumor therapy. Mouse recombinant CXCL4 produced in E. coli is a single, non-glycosylated polypeptide chain containing 73 amino acids and having a molecular mass of 8.2 kDa.
Species
Mouse
Product Overview
Mouse Pf4 expressed in E.coli
Molecular Mass
8.2 kDa
Size
CAT# CSC-CTK0754-20 (20 μg); CAT# CSC-CTK0754-100 (100 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
N/A
Endotoxin Level
N/A
Formulation
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) protein solution in 1X PBS (pH 7.4).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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