Recombinant Murine Endothelial Cell Growth Factor (rMu VEGF) is a homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability. Murine VEGF is a 39.036 kDa homodimeric, non-glycosylated protein consisting of two 165 amino acid polypeptide chains.
Mouse Nrp2 expressed in E.coli
CAT# CSC-CTK0724-10 (10 μg); CAT# CSC-CTK0724-50 (50 μg)
Greater than 95% as determined by SDS-PAGE, FPLC and HPLC analysis.
Recombinant murine VEGF165 is fully biologically active when compared to standards. The biological activity of Creative Bioarrays murine VEGF was determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0 to 5.0 ng/ml.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered, concentrated (1 mg/ml) solution with no additives.
Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized rMu VEGF, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu VEGF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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