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ActoFactor™ Recombinant Mouse Neuropilin 2 (E. coli)

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Cat.No.
CSC-CTK0724
Description
Recombinant Murine Endothelial Cell Growth Factor (rMu VEGF) is a homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability. Murine VEGF is a 39.036 kDa homodimeric, non-glycosylated protein consisting of two 165 amino acid polypeptide chains.
Species
Mouse
Product Overview
Mouse Nrp2 expressed in E.coli
Molecular Mass
39 kDa
Size
CAT# CSC-CTK0724-10 (10 μg); CAT# CSC-CTK0724-50 (50 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE, FPLC and HPLC analysis.
Activity
Recombinant murine VEGF165 is fully biologically active when compared to standards. The biological activity of Creative Bioarrays murine VEGF was determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0 to 5.0 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered, concentrated (1 mg/ml) solution with no additives.
Reconstitution
Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized rMu VEGF, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu VEGF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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