Mouse Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor-dependent cell lines and promotion of megakaryocyte production in vivo. Mouse LIF is a 20 kDa protein containing 181 amino acid residues. The non-glycosylated, E. coli-expressed, recombinant LIF is indistinguishable from native LIF in its biological activity in vitro.
Mouse Lif expressed in E.coli
Mature mouse LIF* was expressed in E. coli as a fusion protein with GST. The GST moiety was cleaved with thrombin and mouse LIF was purified by HPLC chromatography.
CAT# CSC-CTK0056-10 (10 μg); CAT# CSC-CTK0056-50 (50 μg)
Greater than 95% as determined by SDS-PAGE and visualized by silver stain
The activity of recombinant mouse LIF is determined by the ability to induce differentiation of mouse M1 myeloid leukemic cells. The minimum detectable concentration of mouse LIF in this assay is 0.5 ng/mL. The specific activity is 1 x 10^8 units/mg, where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies grown in agar cultures.
Endotoxin level is less than 0.1 ng/mg of mouse LIF.
Supplied at a concentration of 10 μg/mL in phosphate-buffered saline (PBS), pH 7.4, and 0.02% Tween 20. No preservatives are added. Further dilutions should be made into buffer or medium to which protein (e.g. 1% bovine serum albumin) or Tween 20 has been
Storage & Stability
Product stable at 2 - 8°C until expiry date as indicated on the label.
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