Interleukin 6 is a multifunctional cytokine that plays an important role in host defense, acute phase reactions, immune responses and hematopoiesis. IL-6 is expressed by a variety of cell types including T cells, B cells, monocytes/macrophages, fibroblasts, hepatocytes, vascular endothelial cells and various tumor cell lines. IL-6 exerts biological effects on B cells, T cells, hepatocytes and myeloid hematopoietic progenitors. The methionyl form of recombinant mouse IL-6 contains 188 amino acid residues with a predicted molecular mass of approximately a 21.8 kDa.
Mouse Il6 expressed in E.coli
A DNA sequence encoding the mature mouse IL-6 protein was expressed in E. coli.
CAT# CSC-CTK0003-5 (5 μg); CAT# CSC-CTK0003-25 (25 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
The biological activity of recombinant mouse IL-6 is measured in a cell proliferation assay using a mouse factor-dependent plasmacytoma cell line, T1165. The ED50 for this effect is typically 0.02 - 0.06 ng/mL.
Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
Recombinant mouse IL-6 is lyophilized from a 0.2 μm filtered solution in 50 mM sodium acetate, 1 mM EDTA and 5% trehalose, pH 4.0 containing 50 μg bovine serum albumin per 1 μg cytokine.
Reconstitute mouse IL-6 at a concentration greater than 10 μg/mL with sterile phosphate buffered saline (PBS) containing at least 0.1% human or bovine serum albumin.
Storage & Stability
Lyophilized mouse IL-6 is stable for upto twelve months from date of receipt at -20°C to -70°C. Reconstituted mouseIL-6 can be stored under sterile conditions at
2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer)for three months without detectable loss of activity. Avoid repeated freezing and thawing.
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