IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities. Recombinant murine IL-33 is a 17.5 kDa protein containing 158 amino acid residues.
Mouse Il33 expressed in E.coli
CAT# CSC-CTK0704-10 (10 μg); CAT# CSC-CTK0704-50 (50 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
The ED50 as determined by the dose-dependent stimulation of the proliferation of murine D10S cells is ≤ 0.5 ng/ml, corresponding to a specific activity of Greater than 2 x 10^6 units/mg.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered solution in 10 mM sodium phosphate (pH 7.5) and 75 mM NaCl.
Please centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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