Murine IL-25 (SF20) is a bone marrow stroma-derived growth factor. IL-25 is expressed in bone marrow, spleen stroma cells, resting mononuclear cells, resting CD8+ and CD19+ cells and activated CD8+ T cells and has been shown to bind to the surface of cells expressing the receptor TSA-1 (Thymic shared Ag-1). Among its biological activities, IL-25 stimulates the proliferation of FDCP2 cells (a mouse factor-dependent hemopoietic cell line) and mouse lymphoid cells. Recombinant murine IL-25 is a 15.8 kDa protein containing 143 amino acid residues.
Mouse Il25 expressed in E.coli
CAT# CSC-CTK0705-10 (10 μg); CAT# CSC-CTK0705-50 (50 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Less than 1 EU/μg.
Lyophilized (freeze-dried), sterile powder. Lyophilized with no additives.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu IL-25 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
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