Recombinant Murine Interleukin-2 (rMu IL-2) is a potent lymphoid cell growth factor, which exerts its biological activity primarily on T cells. Creative Bioarray's IL-2 is a 17.2 kDa protein containing 149 amino acid residues. It has been purified using proprietary chromatographic techniques.
Mouse Il2 expressed in E.coli
CAT# CSC-CTK0681-20 (20 μg); CAT# CSC-CTK0681-100 (100 μg)
Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.
The ED50, as determined by the dose-dependent stimulation of the proliferation of murine CTLL- 2 cells, is <0.2 ng/ml corresponding to a specific activity of Greater than 5 x 10^6 units/mg.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered, concentrated protein solution (1 mg/ml) in 20 mM PBS (pH 7.4).
Please centrifuge product briefly before opening the vial. The lyophilized rMu IL-2 should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu IL-2 should be stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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