CXCL9 is a chemoattractant for activated T cells having angiostatic activity. Both CXCL9 and CXCL10 have been considered as important components for the anti-tumor activities of interferon-gamma (IFN-gamma) and interleukin-12 in animal models. Human MIG is induced in THP-1 cells and in peripheral blood mononuclear cells by interferon-gamma but not by interferon-alpha or by lipopolysaccharides. Analysis of mouse and human genomic DNAs suggested that the respective human and murine genes are true mouse-human homologues. Recombinant human MIG (monokine induced by gamma-interferon) produced in E. coli is a single, non-glycosylated polypeptide chain of 103 amino acids and a molecular mass of 11,700 Dalton. Recombinant mouse MIG (monokine induced by gamma-interferon) produced in E. coli is a single, non-glycosylated polypeptide chain of 105 amino acids and a molecular mass of 12,208 Dalton.
Mouse Cxcl9 expressed in E.coli
CAT# CSC-CTK0747-20 (20 μg); CAT# CSC-CTK0747-100 (100 μg)
Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Recombinant MIG is fully biologically active when compared to a standard. The biological activity is calculated by its ability to chemoattract human lymphocytes using a concentrations ranging from 0.1-1 ng/ml.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml), sterile solution in water containing no add itives.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu MIG should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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