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ActoFactor™ Recombinant Mouse Chemokine (C-X-C motif) ligand 16

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Cat.No.
CSC-CTK0766
Description
CXCL16 is a member of the CXC chemokine family and signals through the CXCR6 receptor. CXCL16 may play a role in attracting lymphocyte subsets during inflammation and may facilitate certain immune responses. The chemokine domain of CXCL16 contains six cysteine residues including the four highly conserved cysteine residues characteristic of CXC chemokines. The CXCL16 gene codes for a 273 amino acid polypeptide, which includes a 29 amino acid cytoplasmic domain and transmembrane sequence containing approximately 20 amino acids. The extracellular portion of CXCL16 contains a chemokines domain and an extended C-terminal "mucin-like stalk" sequence. The extracellular domain contains 89 amino acid residues (86 a.a. residues for the murine homolog). Recombinant murine CXCL16 is a 9.9 kDa protein containing 88 amino acid residues.
Species
Mouse
Product Overview
Mouse Cxcl16 expressed in E.coli
Molecular Mass
9.9 kDa
Size
CAT# CSC-CTK0766-20 (20 μg); CAT# CSC-CTK0766-100 (100 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by its ability to chemoattract murine lymphocytes using a concentration range of 20- 1000 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered solution with no additives.
Reconstitution
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted CXCL16 should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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