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ActoFactor™ Recombinant Mouse Chemokine (C-X-C motif) ligand 12

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Cat.No.
CSC-CTK0062
Description
Stromal cell-derived factor-1 (SDF-1), also known as Pre-B cell growth stimulating factor (PBSF), is an a family (CXC) chemokine secreted by bone marrow, stromal cells, liver tissue and muscle. SDF-1 is chemoattractant for T lymphocytes, monocytes, pro-and pre-B cells but not neutrophils. SDF-1 was shown to be a ligand for fusin/LESTR, a CXCR4 type receptor that functions as a coreceptor for HIV-1 strains. Recently, SDF-1 was shown to induce lymphocyte homing, mature blood cell functional activation and stem cell growth inhibition. SDF-1 is also a chemoattractant for CD34+ haemopoietic progenitors. Mouse SDF-1a is synthesized as a precursor of 89 amino acid residues processed to the mature form by cleavage of the N-terminus. Recombinant mouse SDF-1a contains 68 amino acid residues and has a predicted molecular mass of 8 kDa.
Species
Mouse
Product Overview
Mouse Cxcl12 expressed in E.coli
Sequence
A DNA sequence encoding the mature mouse SDF-1a protein (aa residues 22-89) was expressed in E. coli.
Size
CAT# CSC-CTK0062-10 (10 μg); CAT# CSC-CTK0062-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
Activity was determined by the ability to chemoattract cultured human lymphocytes and mouse BaF/3 cells transfected with the human CXCR4 receptor. The ED50 using this assay was typically 3 – 9 ng/ml and 0.15 – 0.6 ng/mL, respectively.
Endotoxin Level
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution of 30% acetonitrile, 0.1% TFA containing 50 mg of bovine serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/ml of the cytokine.
Storage & Stability
The lyophilized sampleis stable for greater than sixmonths at -20°C to -70°C. Reconstituted mouse SDF-1 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss ofactivit y. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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