MIP-1a is a member of the b (CC) family of chemokines. It is produced by T cells, B cells and monocytes after antigen or mitogen stimulation. MIP-1a is a chemoattractant for monocytes, CD8+ T cells, B cells, and eosinophils. In addition, MIP-1a acts as a stem cell inhibitory factor suppressing proliferation of
hematopoietic precursors. Mouse MIP-1a cDNA encodes a 92 amino acid residue precursor protein containing a 23 amino acid signal peptide. As reported for other chemokines, cleavage of the amino terminus domain dramatically reduces its activity. MIP-1a binds to CCR1, CCR4 and CCR5 receptors. The 69 amino acid residues recombinant protein has a predicted molecular mass of approximately 7.8 kDa.
Mouse Ccl3 expressed in E.coli
A DNA sequence encoding the mature mouse MIP-1a protein was expressed in E. coli.
CAT# CSC-CTK0031-10 (10 μg); CAT# CSC-CTK0031-50 (50 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined by ability to chemoattract cultured human monocytes or BaF/3 cells transfected with the human CCR5 receptor and the ED50 for the chemoattractant activity was typically 5 - 25 ng/mL or 1.5 - 6 ng/mL, respectively.
Endotoxin level less than 0.1 ng per 1 μg of the cytokine as determined by the LAL method.
Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% TFA, containing 50 μg of bovine serum albumin per 1 μg of cytokine.
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 μg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted mouse MIP-1a/CCL3 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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