Recombinant Human Endocrine Gland-derived Vascular Endothelial Cell Growth Factor (rHu EG- VEGF) is a secreted angiogenetic mitogen growth factor expressed by a variety of vascularized glands. The reported activities of human EG-VEGF include proliferation and migration in capillary endothelial cells derived from endocrine glands. RHu EG-VEGF is a 9.6 kDa protein consisting of 86 amino acid residues.
Human VEGFA expressed in E.coli
CAT# CSC-CTK0393-20 (20 μg); CAT# CSC-CTK0393-100 (100 μg)
Greater than 97% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
The activity as determined by the dose-dependent proliferation of MIA PaCa-2 cells is typically 1- 4 μg/ml.
Less than 1 EU/μg.
Sterile-filtered and lyophilized from a concentrated (1 mg/ml) solution with no additives.
Please centrifuge product briefly before opening the vial. The lyophilized human EG-VEGF should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu EG-VEGF should be stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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