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ActoFactor™ Recombinant Human Vascular endothelial cell growth factor 121 (E. coli)

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Cat.No.
CSC-CTK0389
Description
Recombinant Human Vascular Endothelial Cell Growth Factor121 (rHu VEGF-121) is a 28.4 kDa homodimeric protein consisting of two non-glycosylated 121 amino acid polypeptide chains. VEGF-121 is a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF-121 include stimulation of endothelial cell growth, angiogenesis, and capillary permiability. VEGF-121 does not bind on heparin.
Species
Human
Product Overview
Human VEGFC expressed in E.coli
Molecular Mass
28.4 kDa
Size
CAT# CSC-CTK0389-10 (10 μg); CAT# CSC-CTK0389-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE.
Activity
The ED50 as determined by (3H)-thymidine incorporation into human umbilical vein endothelial (HUVE) cells and stimulation of cell proliferation was found to be at a concentration of 0.2-0.4 ng/ml corresponding to a specific activity of 2.5 - 5 x 10^6 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a concentrated protein solution (1 mg/ml) with no additives.
Reconstitution
Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration not lower than 50 μg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage & Stability
Lyophilized cytokine is stable as shipped for at least 1 year at 20°C to 70°C. Reconstituted protein should be stored in working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Customer Support & Price Inquiry
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