TNFR binds specifically to tumor necrosis factor (TNF) and blocks its interaction with cell surface TNF receptors. TNF is a naturally occurring cytokine that is involved in normal inflammatory and immune responses. It plays an important role in the inflammatory processes of rheumatoid arthritis (RA), polyarticular-course juvenile rheumatoid arthritis (JRA) and ankylosing spondylitis. In addition, TNF plays a role in the inflammatory process of plaque psoriasis. Elevated levels of TNF are found in involved tissues and fluids of patients with RA, psoriatic arthritis, ankylosing spondylitis (AS) and plaque psoriasis. Recombinant TNFR inhibits the activity of TNF in vitro and has been shown to affect several animal models of inflammation, including murine collagen- induced arthritis. It inhibits binding of both TNF-α and TNF-β (lymphotoxin alpha [LTα]) to cell surface TNFRs, rendering TNF biologically inactive. Cells expressing transmembrane TNF that bind to TNFR are not lysed in vitro in the presence or absence of complement. rTNFR can also modulate biological responses that are induced or regulated by TNF, including expression of adhesion molecules responsible for leukocyte migration (i.e., E-selectin and to a lesser extent intercellular adhesion molecule-1 [ICAM-1]), serum levels of cytokines (e.g., IL-6) and serum levels of matrix metalloproteinase-3 (MMP-3 or stromelysin).|Human TNFR produced in CHO is a dimeric, glycosylated polypeptide chain consisting of the extracellular ligand-binding portion of the human 75 kDa (p75) tumor necrosis factor receptor (TNFR) linked to the Fc portion of human IgG1. The Fc component of TNFR contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It consists of 934 amino acids and has an apparent molecular weight of approximately 150 kDa.
Human TNFRSF10B expressed in CHO cells
CAT# CSC-CTK0348-50 (50 μg); CAT# CSC-CTK0348-250 (250 μg)
Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Recombinant human TNFR is fully biologically active when compared to a standard. Potency is determined by its ability to neutralize TNF-alpha mediated growth inhibition of A375 cells, corresponding to a specific activity of 1.7 x 10^7 IU/mg.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried) powder. Each mg contains 1.6 μg mannitol, 0.4 μg sucrose and 48 μg tromethamine.
Please centrifuge product briefly before opening the vial. The lyophilized rHu TNFR should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu TNFR should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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