Human BAFF (B cell Activating Factor) is a ligand in the tumor necrosis factor (TNF) family and is also known as B Lymphocyte Stimulator (BLyS). It binds to several receptors including the BAFF receptor, BCMA and TACI, the latter two are shared with the ligand APRIL. It is known as a co-stimulator of B cell proliferation and has been described together with APRIL as important for the proliferation of human embryonic stem cells. Recombinant Human BAFF is a single, non-glycosylated polypeptide chain containing 153 amino acids and has a MW of 17.0 kDa.
Human TNFSF13B expressed in E.coli
Expressed in E. Coli.
CAT# CSC-CTK0061-20 (20 μg); CAT# CSC-CTK0061-100 (100 μg)
Greater than 95% as determined by SDS-PAGE and visualized by silver stain
The ED50 of BAFF, as determined by the dose-dependant stimulation of IL-8 production by human PBMC, is generally less than 10 ng/mL. Results may vary with PBMC donors.
The endotoxin level is less than 0.1 ng/μg of BAFF.
Lyophilized from 0.3X phosphate buffered saline at pH 7.5.
Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL.
Storage & Stability
Lyophilized BAFF is best-stored desiccated below 0°C. Reconstituted BAFF should be stored in working aliquots at -20°C. Avoid repeated freezing and thawing.
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