Recombinant human soluble RANKL produced in E. coli is a single, non-glycosylated polypeptide chain of 176 amino acids and a molecular mass of 20,006 Dalton.
Human TNFSF11 expressed in E.coli
CAT# CSC-CTK0295-10 (10 μg); CAT# CSC-CTK0295-50 (50 μg)
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
RANK ligand is fully biologically active when compared to a standard. The activity as determined by its ability to induce a dose-dependent stimulation of IL-8 production in human PBMCs is 39- 51 ng/ml.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried)powder. Recombinant soluble RANK ligand was lyophilized from a concentrated (1 mg/ml) solution containing 10 mM sodium phosphate (pH 8.0).
Please centrifuge product before briefly opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu sRANKL should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.