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ActoFactor™ Recombinant Human Tumor necrosis factor
- Specification
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Cat.No.
CSC-CTK0008
Description
Tumor Necrosis Factor - alpha (TNF-a) occurs as a secreted, soluble form and as a membrane anchored form, both of which are biologically active. TNF-a is a pleiotropic factor activating multiple signal transduction pathways and inducing or suppressing the expression of a wide variety of genes. TNF-a plays a critical role in mediation of the inflammatory response and in influencing resistance to infections and tumor growth. Recombinat human TNF-a is a 17.5 kDa protein containing 158 amino acid residues.
Species
Human
Product Overview
Human TNF expressed in E.coli
Sequence
A DNA sequence encoding mature human TNF-a was expressed in E. coli.
Size
CAT# CSC-CTK0008-10 (10 μg); CAT# CSC-CTK0008-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The biological activity of recombinant TNF-a was measured in a cytotoxicity assay using the TNF-susceptible L-929 cell line in the presence of the metabolic inhibitor, actinomycin D. The ED50 for this effect is typically 0.02 - 0.05 ng/mL.
Endotoxin Level
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/ml of the cytokine.
Storage & Stability
The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted TNF-a can be stored under sterile conditions at 2°C to 4°C for one month or at -20 °C to -70 °C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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