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ActoFactor™ Recombinant Human Trefoil factor 1

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Cat.No.
CSC-CTK0421
Description
The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF1 is essential for normal differentiation of the antral and pyloric gastric mucosa and functions as a gastric-specific tumor suppressor gene. Recombinant human TFF1 is a 13.2 kDa homodimeric protein consisting of two 60 amino acid polypeptide chains, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
Species
Human
Product Overview
Human TFF1 expressed in E.coli
Molecular Mass
13.2 kDa
Size
CAT# CSC-CTK0421-20 (20 μg); CAT# CSC-CTK0421-100 (100 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by HPLC and SDS-PAGE analysis.
Activity
Determined by its ability to activate ERK1/2 (a MAPkinase signaling molecule) using a concentration 1.0-2.0 μg/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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