SOX-2 is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES-1, FGF-4, UTF-1 and ZFP-206. SOX-4 is important for early embryogenesis and for embryonic stem cell pluripotency and plays also an important role for the determination of cell fate. Recombinant human SOX-2 produced in E. coli is a single, non-glycosylated polypeptide chain of 208 amino acids (1-200 a.a.) and a molecular mass of 23.1 kDa. SOX-2 protein is fused to an 8 amino acid His-tag at the C-terminus and purified by standard chromatography.
Human SRY expressed in E.coli
CAT# CSC-CTK0643-25 (25 μg); CAT# CSC-CTK0643-125 (125 μg)
Greater than 90% as determined by SDS-PAGE analysis.
Less than 1 EU/μg.
Sterile filtered, colorless solution containing 20 mM Tris-HCl (pH 8.0), 2 mM DTT, 0.2 M NaCl and 40% glycerol.
Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
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