Human Apo-SAA is a 104 amino acid polypeptide that circulates primarily in association with high-density lipoproteins (HDL). The level of apo-SAA, normally 1-5 μg/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL apo-lipoprotein. The human SAA gene codes for a 122 amino acid polypeptide, which contains an 18 amino acid N-terminal signal sequence. Recombinant Apo- SAA is a consensus SAA molecule corresponding to human apo-SAA1alpha except for the presence of an N-terminal methionine and substitution of asparagine for aspartic acid at position 60 and arginine for histidine at position 71(the later two substituted residues are present in apo- SAA2beta).
Human SAA1 expressed in E.coli
CAT# CSC-CTK0611-50 (50 μg); CAT# CSC-CTK0611-250 (250 μg)
Greater than 98% as determined by HPLC and SDS-PAGE analysis.
For most in vitro applications, Apo-SAA exerts its biological activity in the concentration range of 10-50 μg/ml.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered solution with no additives.
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted Apo-SAA should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
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