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ActoFactor™ Recombinant Human Prolactin receptor (GST-tagged)

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Cat.No.
CSC-CTK0103
Description
Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription. Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function. The Prolactin Receptor is fused with a GST tag and has been purified using chromatographic techniques.
Species
Human
Product Overview
Human PRLR expressed in E.coli
Molecular Mass
45 kDa
Size
CAT# CSC-CTK0103-5 (5 μg); CAT# CSC-CTK0103-25 (25 μg)
Expression System
E.coli
Purity
N/A
Activity
N/A
Endotoxin Level
N/A
Formulation
Sterile-filtered, liquid. 100 μl purified recombinant human PRLR protein (amino acids 432-623) at 0.1 mg/ml in 50 mM Tris-Acetate (pH7.5), 1 mM EDTA and 20% glycerol.
Reconstitution
Please centrifuge product briefly before opening vial. The solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
rHu Prolactin Receptor should be used immediately or can be stored for up to 12 months in undiluted working aliquots at -20°C to –80°C. Avoid repeated freeze-thaw cycles. Applications The Prolactin Receptor can be used for ELISA and Western Blot studies as well as for inhibition assays. On SDS-PAGE Commassie blue-stained gel, the purified recombinant protein shows a band at 45 kDa. The biological activity has not been determined.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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