Prolactin (PRL) is a neuroendocrine pituitary hormone. PRL is synthesized by the anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, NK cells and breast cancer cells. PRL is a lactogenic hormone, also involved in breast cancer development , regulation of reproductive function,
and immunoregulation. In the immune system, PRL has been shown to be secreted by human PBMC and to act as a proliferative growth factor and an IFN-g inducer. The mature protein containing 200 amino acid residues migrates with an apparent Mr of 23 kDa. PRL binds to a class I cytokine receptor. Prolactin signal transduction involves the JAK/STAT families and Src kinase family.
Human PRL expressed in E.coli
A DNA sequence encoding the mature human prolactin protein sequence (aa residues 29 - 227) was expressed in E. coli.
CAT# CSC-CTK0060-50 (50 μg); CAT# CSC-CTK0060-250 (250 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined in a cell proliferation assay using the rat lymphoma, Nb2-11 and the ED50 for this effect was typically 0.03 - 0.1 ng/mL.
Endotoxin level less than 0.1 ng per 1mg of the cytokine as determined by the LAL method.
Lyophilized from a 0.2 μm filtered solution in 30% CH3CN, 0.1% TFA.
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/mL.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted PRL can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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