Recombinant Human Platelet Derived Growth Factor (rHu PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-BB produced in E. coli is a 24.3 kDa B chain homodimer formed by subunits of 109 amino acid residues. It has been purified using proprietary chromatographic techniques.
Human PDGFB expressed in E.coli
CAT# CSC-CTK0281-10 (10 μg); CAT# CSC-CTK0281-50 (50 μg)
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
The ED50, as determined by the dose-dependent stimulation of the proliferation of murine Balb/c 3T3 cells, is 1.0-3.0 ng/ml.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered, concentrated protein solution in PBS (pH 7.4) without additives.
Please centrifuge product before briefly opening vial. The lyophilized PDGF-BB should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted PDGF-BB should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0,1% HSA or BSA). Please avoid repeated freeze / thaw cycles.
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