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ActoFactor™ Recombinant Human Pigment Epithelium Derived Factor (HEK)
- Specification
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Cat.No.
CSC-CTK0286
Description
PEDF is a neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Aqueous humour level of asymmetric dimethylarginine is correlated with PEDF in humans. ADMA and PEDF levels are increased in response to inflammation in uveitis. Lack of PEDF expression is a potent factor for the enhancement of tumor growth and angiogenesis in breast cancer. PEDF & VEGF genes contribute to the development of diabetic retinopathy. PEDF and VEGF structural changes in blood vessel wall play an important role in the pathophysiology of PD patients. PEDF-overexpressing tumors exhibited reduced intratumoral angiogenesis. Levels of the natural ocular anti-angiogenic factor SentrinF1 (PEDF) is associated with proliferative retinopathy. VEGF secreted by retinal pigment epithelial cells upregulates PEDF expression via VEGFR-1 in an autocrine manner. Sentrin-F1 concentration in the aqueous humor of diabetic patients predicts who will develop progression of retinopathy. PEDF blocks angiogenic effects of leptin through its anti-oxidative properties. Human recombinant PEDF produced in HEK cells is a single, glycosylated, polypeptide chain containing 410 amino acids and having a molecular mass of 45.6 kDa. The Human Sentrin-F1 is fused to FLAG tag at N-Terminus.
Species
Human
Product Overview
Human SERPINF1 expressed in HEK cells
Molecular Mass
N/A
Size
CAT# CSC-CTK0286-10 (10 μg); CAT# CSC-CTK0286-50 (50 μg)
Expression System
HEK cells
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
N/A
Endotoxin Level
N/A
Formulation
The filtered concentrated (0.5 mg/ml) protein solution was lyophilized against 20 mM Tris and 20 mM NaCl (pH 7.5).
Reconstitution
Please centrifuge product briefly before opening vial. It is recomnded to add deionized water to a working concentration of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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