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ActoFactor™ Recombinant Human Neurotrophin 4

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Cat.No.
CSC-CTK0488
Description
NT-4 is a neurotrophic factor structurally related to β-NGF, BDNF, and NT-3. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-4 is expressed in the prostate, thymus, placenta and skeletal muscle. NT-4 can signal through the LNGFR and trkB receptors and promotes the survival of peripheral sensory sympathetic neurons. Recombinant human NT-4 is a noncovalently linked homodimer, of two 14.0 kDa polypeptide monomers (260 total amino acid residues).
Species
Human
Product Overview
Human NTF4 expressed in E.coli
Molecular Mass
28.1 kDa
Size
CAT# CSC-CTK0488-10 (10 μg); CAT# CSC-CTK0488-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures, it was found to be in the range of 20-50 ng/ml corresponding to 2 x 10^4 U/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered solution with no additives.
Reconstitution
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted NT-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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