Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up- regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-2 is a secreted collagenase with specificity toward Type IV, V, VII, and X collagens. Recombinant human MMP-2 produced in HEK293 cells is a proform of the Human MMP-2 (Ala30-Cys660) and fused with a ployhistide tag at the C-terminus, having a MW of 71kDa.
Human MMP2 expressed in HEK cells
CAT# CSC-CTK0369-10 (10 μg); CAT# CSC-CTK0369-50 (50 μg)
Greater than 95% as determined by SDS-PAGE.
The activity was measured by its ability to cleave the colorimetric peptide substrate Mca-PLGL- DpaAR-NH2. The specific activity is > 1,000 pmoles/min/μg.
Less than 1 EU/μg.
The MMP-2 is supplied as a 0.2μm filtered solution in Tris, NaCl and Brij35.
Please centrifuge product briefly before opening vial. The protein solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. Can also be used as standard in Western blot, etc.
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