Recombinant Human TNF-beta (Lymphotoxin) produced in E. coli is a single, non-glycosylated, polypeptide chain of 172 amino acids and having a molecular mass of 18,645 Dalton.
Human LTA expressed in E.coli
CAT# CSC-CTK0349-20 (20 μg); CAT# CSC-CTK0349-100 (100 μg)
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
TNF-beta is fully biologically active when compared to a standard. The ED50, as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D, is less then 0.05 ng/ml corresponding to a specific activity of 2 ×10^7 IU/mg.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized protein with no additives.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu TNF-beta should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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