Featured Products
- HCC-78
- HDLM-2
- DOHH-2
- L-540
- MX-1
- NALM-6
- NB-4
- CAL-51
- SNB-19
- KYSE-520
- MKN-45
- BA/F3
- MS-5
- HCEC-B4G12
- NK-92
- PA-TU-8988S
- MONO-MAC-1
- PA-TU-8902
- Human Microglia
- Human Hepatic Stellate Cells
- Human Skeletal Muscle Cells (DMD)
- Human Schwann Cells
- Human Oral Keratinocytes (HOK)
- Human Cardiomyocytes
- Human Small Intestinal Epithelial Cells
- Human Colonic Epithelial Cells
- Human Intestinal Fibroblasts
- Primary Human Large Intestine Microvascular Endothelial Cells
- Human Small Intestinal Microvascular Endothelial Cells
- Human Retinal Pigment Epithelial Cells
- Human Hepatocytes
- Cynomolgus Monkey Lung Microvascular Endothelial Cells
- Cynomolgus Monkey Vein Endothelial Cells
- C57BL/6 Mouse Primary Mammary Epithelial Cells
- C57BL/6 Mouse Vein Endothelial Cells
- Rat Primary Kidney Epithelial Cells
- Rat Gingival Epithelial Cells
- Rabbit Lung Endothelial Cells
Our Promise to You
Guaranteed product quality, expert customer support
ActoFactor™ Recombinant Human Leukemia inhibitory factor
- Specification
- Recommended Products
Cat.No.
CSC-CTK0033
Description
Leukemia inhibitory factor (LIF) is a pleiotropic cytokine produced by many different cell and tissue types including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanomas. LIF stimulates the differentiation of the macrophage cell line M1, and the proliferation of hematopoietic stem cells. In vivo, it has profound effects on hematopoiesis, particularly in combination with other cytokines such as IL-3, causing increased platelet formation.
LIF allows embryonic stem cells to remain in an undifferentiated state and can maintain their proliferation in culture. LIF stimulates synthesis of acute phase proteins by liver cells, increases bone resorption, stimulates differentiation of cholinergic nerves and loss of body fat. The mature protein contains 180 amino acid residues, several potential glycosylation sites and three disulfide bonds. LIF binds to a heterodimeric receptor formed by an α binding chain and gp130.
Species
Human
Product Overview
Human LIF expressed in E.coli
Sequence
Human LIF was expressed in E. coli as a fusion protein with GST. The GST moiety was cleaved with thrombin and human LIF was purified by HPLC.
Size
CAT# CSC-CTK0033-10 (10 μg); CAT# CSC-CTK0033-50 (50 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and visualized by silver stain
Activity
The biological activity of recombinant human LIF was measured by the ability to induce differentiation of murine M1 myeloid leukemic cells. The minimal detectable concentration of human LIF in this assay is 0.5 ng/mL. The specific activity is 1 x 10^8 units/mg, where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 colonies in 1 ml agar cultures.
Endotoxin Level
Endotoxin level is less than 0.1 ng/mg of human LIF as determined by the Limulus Amoebocyte Lysate (LAL) method.
Formulation
Supplied at a concentration of 10 μg/mL in phosphate-buffered saline (PBS), pH 7.4 and 0.02% Tween 20. Further dilutions should be made into buffer or medium to which protein (e.g. 1% bovine serum albumin) or Tween 20 has been added.
Storage & Stability
Product stable at 2 - 8°C until expiry date as indicated on the label.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Customer Support & Price Inquiry
