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ActoFactor™ Recombinant Human Leptin Binding Domain

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Cat.No.
CSC-CTK0607
Description
Human recombinant Leptin soluble Receptor (also called Leptin Binding Domain) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa. Leptin Receptor consists of the cytokine binding domain of leptin receptor amino acids 428-635 of human Leptin Receptor. The Leptin Binding Domain has been purified by proprietary chromatographic techniques.
Species
Human
Product Overview
Human LEP expressed in E.coli
Molecular Mass
24.5 kDa
Size
CAT# CSC-CTK0607-20 (20 μg); CAT# CSC-CTK0607-100 (100 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and SEC-HPLC analysis.
Activity
N/A
Endotoxin Level
N/A
Formulation
Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO .
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized Leptin Binding Domain is very soluble in water and most aqueous buffers below and above the isoelectric point. The lyophilized protein should be reconstituted in sterile, ultrapure water or aqueous buffers to a concentration of 0.1 - 1.0 mg mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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