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ActoFactor™ Recombinant Human KIT ligand

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Cat.No.
CSC-CTK0291
Description
Stem Cell Factor (SCF, also known as c-kit ligand, mast cell growth factor and steel factor) is a hematopoietic growth factor acting at the early stages of hematopoiesis. SCF promotes the survival, proliferation and differentiation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also stimulates the growth and activation of mast cells and eosinophils. SCF has been shown to act synergistically with other growth factors, such as GM- CSF, G-CSF, IL-7 and EPO. Human SCF shows low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant human SCF is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E. coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture. Recombinant human SCF contains 165 amino acids and a 16 a.a. histidine-based tag for a total length of 181 a.a. and has a predicted molecular mass of 20.7 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 22-24 kDa in SDS-PAGE.
Species
Human
Product Overview
Human KITLG expressed in Endosperm tissue of barley grain (Hordeum vulgare)
Molecular Mass
20.7 kDa
Size
CAT# CSC-CTK0291-10 (10 μg); CAT# CSC-CTK0291-50 (50 μg)
Expression System
Endosperm tissue of barley grain (Hordeum vulgare)
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
Bioactivity is assayed by measuring the dose-dependent effect of human SCF on the proliferation of TF-1 cells. The ED50 for this batch of recombinant human SCF was determined to be 4.5 ng/ml, corresponding to specific activity of 0.22 x 10^6 U/mg. We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay.
Endotoxin Level
Less than 0.05 EU/μg.
Formulation
Sterile filtered through a 0.2 μm filter. Lyophilized from 30 mM Na-acetate, 0.7 M NaCl (pH 4.5).
Reconstitution
Please centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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