Recombinant Human Interleukin-9 (rHu IL-9) is a T-cell derived growth factor produced preferentially by CF4+ helper cells. rHu IL-9 is a pleiotropic cytokine with multiple functions on cells of lymphoid, myeloid, and mast cell lineages. It is thought to be a regulator of hematopoiesis and has been shown to enhance the growth of human mast cells and megakaryoblastic leukemic cells as well as murine helper T-cell clones. IL-9 is a glycoprotein with a molecular weight of 32-39 that is derived from T-cells, and maps to human chromosome 5. Human IL-9 produced in HEK cells is a glycosylated monomer, having a molecular weight range of 38-48 kDa due to glycosylation. It has been purified by proprietary chromatographic techniques.
Human IL9 expressed in HEK cells
CAT# CSC-CTK0242-10 (10 μg); CAT# CSC-CTK0242-50 (50 μg)
Greater than 95% as determined by SDS-PAGE analysis.
rHu IL-9 is fully biologically active when compared to standards. The specific activity was determined by the dose-dependent stimulation of the proliferation of human MO7e cells (human megakaryoblastic leukemia cell line) and is typically 0.03-0.2 ng/ml.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried)powder. Lyophilized from a concentrated protein solution (1 mg/ml) in 1xPBS.
Please centrifuge product briefly before opening vial. The lyophilized rHu IL-9 should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-9 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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