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ActoFactor™ Recombinant Human Interleukin 8

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Cat.No.
CSC-CTK0380
Description
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins which include NP-1, NP-2 and NP-3, are distinguished from the beta-defensins by the pairing of their three disulfide bonds. In addition to antimicrobial activity, NP-1 exhibits chemotactic activity on dendritic cells. NP-1 is expressed as the C-terminal portion of an inactive precursor protein, which also contains a 19 amino acid N-terminal signal sequence and a 45 amino acid polypeptide. NP-1 contains a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human NP-1 is a 3.4 kDa protein containing 30 amino acid residues.
Species
Human
Product Overview
Human IL8 expressed in E.coli
Molecular Mass
3.4 kDa
Size
CAT# CSC-CTK0380-20 (20 μg); CAT# CSC-CTK0380-100 (100 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
Determined by its ability to chemoattract immature dendritic cells using a concentration of 1.0- 10.0 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized (freeze-dried) powder.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu NP-1 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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