Recombinant Human Interleukin-7 (rHu IL-7) is a potent lymphoid cell growth factor, which affects Pre-B, Pro-B and early T-cells. It will also affect mature T-cells in combination with other factors such as IL2. |Human recombinant Interleukin-7 produced in yeast is a single, glycosylated polypeptide chain of 152 amino acids having a molecular mass of 17.4 kDa. It has been purified by proprietary chromatographic techniques.
Human IL7 expressed in Saccharomyces cerevisiae
CAT# CSC-CTK0237-10 (10 μg); CAT# CSC-CTK0237-50 (50 μg)
Greater than 98% as determined by SDS-PAGE analysis.
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by murine pre- B cell line 2E8 is 2 x 10^6 units/mg.
Lyophilized from a concentrated (1 mg/ml), sterile-filtered protein solution in 20 mM phosphate buffer.
Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at –20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted rHu IL-7 should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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