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ActoFactor™ Recombinant Human Interleukin 6 receptor (HEK)

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Cat.No.
CSC-CTK0233
Description
IL-6 mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6R alpha and gp130. The IL-6R alpha chain is the binding component specific to IL-6; while the gp130 only transmits signals of IL-6 when bound to IL-6R alpha. The gp130 also can transmit signals from LIF, OSM, CNTF, IL-11 and CT-1 in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6R alpha alone. IL-6R alpha is expressed in a wide range of cells including T cells, fibroblasts and macrophages. Soluble IL-6R alpha which consists of only the extracellular domain of the IL-6R alpha chain, acts as an agonist of IL-6 activity at low concentrations. Recombinant human sIL-6R produced in E. coli is a 37.6 kDa protein consisting of the extracellular domain of the IL-6Rα chain (339 amino acid residues).
Species
Human
Product Overview
Human IL6R expressed in HEK293 cells
Molecular Mass
37.6 kDa
Size
CAT# CSC-CTK0233-20 (20 μg); CAT# CSC-CTK0233-100 (100 μg)
Expression System
HEK293 cells
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by its ability to intensify the IL-6 induced growth inhibition of mouse M1 cells. The expected ED50 is ≤ 5.0 ng/ml, in the presence of 20 ng/ml of rhIL-6.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile filtered through a 0.2 micron filter and lyophilized from 1x PBS (pH 7.2).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile 1XPBS (pH 7.2) to a concentration of 0.1 -1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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