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ActoFactor™ Recombinant Human Interleukin 6 (interferon, beta 2)

  • Specification
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Human IL-6 is an important pro-inflammatory and anti-inflammatory cytokine expressed by many cell types including: T and B cells, macrophages, endothelial cells, fibroblasts, monocytes, keratinocytes and certain tumor cells. It Is a multifunctional cytokine that modulates several physiologic processes such as hematopoiesis, stimulation of immunoglobulin synthesis, maturation and activated B cells, differentiation of T lymphocytes and regulation of the hepatic acute-phase response. IL-6 is also produced by muscle, is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. The binding of IL-6 to its receptor triggers the association of a hetheromeric IL-6R/gp130 complex, which activates the JAK/STAT1 cascade. In a mechanism known as trans-signaling, the complex of soluble IL-6/IL-6R elicits responses from gp130-expressing cells that lack cell surface IL-6R. This enables a wider range of cell types to respond to IL-6. Recombinant human Interleukin-6 is a polypeptide chain containing 183 amino acids (30–212 of P05231 IL6_HUMAN) and a 10XHistidine-based tag. It has a predicted molecular mass of 22.2 kDa. However, as a result of potential glycosylation, the recombinant protein could migrate with an apparent molecular mass of 23-24 kDa in an SDS-PAGE gel. Human recombinant IL-6 expressed in Nicotiana benthamiana is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product does not contain any animal–derived components or impurities.
Product Overview
Human IL6 expressed in Nicotiana benthamiana
Molecular Mass
22.2 kDa
CAT# CSC-CTK0231-20 (20 μg); CAT# CSC-CTK0231-100 (100 μg)
Expression System
Nicotiana benthamiana
Greater than 97% as determined by SDS-PAGE analysis.
rHu IL-6 is fully biologically active when compared to standards. The specific activity is determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erytroleukemic indicator cell line). The measured ED50 is typically less than 1 ng/ml.
Endotoxin Level
Less than 0.04 EU/μg.
Lyophilized from a concentrated, sterile protein solution in 20 mM Tris-HCl buffer (pH 8.5) and 125 mM NaCl.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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