IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells. Recombinant human IL-4 produced in plant contains 130 amino acids and a 16 a.a. histidine- based tag for a total length of 146 a.a., and has a predicted molecular mass of 17.2 kDa. The recombinant protein migrates as two bands with an apparent molecular mass of 23 kDa and 24 kDa in SDS-PAGE. recombinant human IL-4 is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.
Human IL4 expressed in Endosperm tissue of barley grain (Hordeum vulgare)
CAT# CSC-CTK0224-10 (10 μg); CAT# CSC-CTK0224-50 (50 μg)
Endosperm tissue of barley grain (Hordeum vulgare)
Greater than 95% as determined by SDS-PAGE analysis.
Each batch is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of recombinant human IL-4 was determined by its dose-dependent effects on the proliferation of TF-1 cells. The ED50 value for this effect is typically ~0.2 ng/ml, corresponding to a specific activity of ~5 x 10^6 units/mg. Optimal concentration should be determined for specific applications and cell lines.
Less than 0.05 EU/μg.
Sterile filtered through a 0.2 μm filter. Lyophilized from PBS (pH 7.2).
Please centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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