Human IL-32 alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-α, and MIP-2 production from human & mouse macrophage cell lines. It is up-regulated in activated T- & NKcells, and IFN-γ-treated epithelial cells. Human recombinant IL-32 alpha produced in E. coli is a 131 amino acid non-glycosylated polypeptide with a molecular mass of 14.9 kDa.
Human IL32 expressed in E.coli
CAT# CSC-CTK0274-10 (10 μg); CAT# CSC-CTK0274-50 (50 μg)
Greater than 97% as determined by SDS-PAGE.
Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED50 for this assay is in the range of 0.125-1.0 μg/ml.
Less than 1 EU/μg.
Lyophilized from a protein solution containing 50 mM NaP, pH 7.5.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-32 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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