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ActoFactor™ Recombinant Human Interleukin 2 (HEK)

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Cat.No.
CSC-CTK0218
Description
IL-2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. Recombinant Human Interleukin-2 (rHu IL-2) produced in HEK cells is a glycosylated monomer, having a total molecular weight of 15 kDa. It has been purified by proprietary chromatographic techniques.
Species
Human
Product Overview
Human IL2 expressed in HEK cells
Molecular Mass
15 kDa
Size
CAT# CSC-CTK0218-10 (10 μg); CAT# CSC-CTK0218-50 (50 μg)
Expression System
HEK cells
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
rHu IL-2 is fully biologically active when compared to standards. The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line) and is typically 0.2-2 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered, white, lyophilized (freeze-dried)powder. Lyophilized from a concentrated protein solution (1 mg/ml) in 1xPBS.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-2 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze/thaw cycles. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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