Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes and macrophages. It is essential for proper immune response, it may also be an important factor in the natural suppression of autoimmunity. IL-2 may be of use as an anti-tumor agent in cancer therapy. Human and mouse IL-2 share 56% aa sequence identity and exhibit cross-species activity. Recombinant human IL-2 produced in plant contains 133 amino acids and a 16 a.a. histidine- based tag for a total length of 149 a.a., and has a predicted molecular mass of 17.5 kDa. The recombinant protein migrates with an apparent molecular mass of 22 kDa in SDS-PAGE. Recombinant human IL-2 is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.
Human IL2 expressed in Endosperm tissue of barley grain (Hordeum vulgare)
CAT# CSC-CTK0217-50 (50 μg); CAT# CSC-CTK0217-250 (250 μg)
Endosperm tissue of barley grain (Hordeum vulgare)
Greater than 95% as determined by SDS-PAGE analysis.
Each batch is tested for bioactivity and verified to have comparable activity to a commercial source. Bioactivity of recombinant human IL-2 is assayed by measuring its dose-dependent effect on proliferation of CTLL-2 cells. The ED50 for this effect is typically 1 x 10^6 units/mg. Optimal concentration should be determined for specific applications and cell lines.
Less than 0.05 EU/μg.
Sterile filtered through a 0.2 μm filter. Lyophilized from PBS (pH 7.2).
Please centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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