IL-17F, a member of the IL-17 family of structurally related cytokines, has been shown to stimulate proliferation and activation of T-cells and PBMCs. IL-17F shares sequence similarity with IL-17. IL-17F is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL-6, IL-8, and CSF2/GM-CSF. IL-17F inhibits the angiogenesis of endothelial cells and induce endothelial cells to produce IL-2, TGFB1/TGFB, and monocyte chemoattractant protein-1. IL-17F induces stromal cells to produce proinflammatory and hematopoietic cytokines. Intestinal IL-17F gene expression is increased in active CD. IL-17A and IL-17F alleles influence the susceptibility to and pathophysiological features of ulcerative colitis independently. IL-17F and MIF gene polymorphisms are significantly associated with the development of functional dyspepsia. The initiation of IL-17F/IL-17R signaling pathway requires the receptor ubiquitination by TRAF-6. IL-17F induces expression of IFN-gamma-inducible protein 10 (IP-10) by activating Raf1- mitogen-activated protein kinase 1/2-extracellular-regulated kinase 1/2-p90 ribosomal S6 kinase-cyclic AMP response element-binding protein signaling pathway.IL-17F also regulates cartilage matrix turnover and inhibits angiogenesis. Recombinant human IL-17F produced in HEK cells is a glycosylated homodimer, having a total molecular weight of 38 kDa. It has been purified by proprietary chromatographic techniques.
Human IL17F expressed in HEK cells
CAT# CSC-CTK0263-10 (10 μg); CAT# CSC-CTK0263-50 (50 μg)
Greater than 95% as determined by SDS-PAGE analysis.
rHu IL-17F is fully biologically active when compared to standards. The specific activity was determined by the dose-dependent induction of IL-6 secretion from NHDF adult fibroblasts, and is typically 100-500 ng/ml.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried)powder. Lyophilized from a concentrated protein solution (1 mg/ml) in 1xPBS.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-17F should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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